INVERTASE BIOSYNTHESIS BY SACCHAROMYCES CEREVISIAE USING CANE MOLASSES

Abstract

Invertase biosynthesis in Saccharomyces cerevisiae OJK32 cells after alcohol fermentation using cane molasses has been carried out. At TRS concentration of 4%, a maximum degree of intracellular invertase production (1 40-150 Elg) has been reached for 6 hours. The sugars induction effect in molasse medium is registered to the 3% concentration in case of using for invertase biosynthesis the strain only. A two-stage culture of the strain examined has been carried out in a bioreactor and a four times increase of intracellular invertase activity has been reached.