Transferability of Some Nuclear Microsatellite (SSRs) Markers from Related Species in Parkia biglobosa.

Abstract

Molecular DNA markers, particularly microsatellites (SSRs), are important tools for plant genetic resources characterization. The present study examined the transferability of nine polymorphic microsatellite markers developed for Parkia panurensis in Parkia biglobosa from Northern Benin, favorite area of Parkia biglobosa. Fourty (40) accessions have been considered. Five microsatellite loci (Parpan 3, Parpan 4, Parpan 9, Parpan 13 and Parpan 15) showed good amplifications in Parkia biglobosa. The size of the amplified markers, ranging from 100 to 200 bases pairs, was similar to those previously reported. Forest tree species are too numerous to benefit all a significant investment in molecular biology. Also, the present study has shown that the transfer of markers between species is possible. The transferability is possible for the species Parkia biglobosa, too. The microsatellites identified in this study are important to analyse the genetic structure and diversity of natural populations of Parkia biglobosa in Benin particularly and in the world generally. They are also important to analyse the evolution of species with phylogeny construction, to analyse the genome with its applications in genetic improvement and genetic identification of given material. For these different studies, the microsatellites of the present study could be completed to those defined for Parkia biglobosa.