Eradication of Cassava (Manihot esculenta)Mosaic Symptoms through Thermotherapy and Meristems Cultured in vitro.

Abstract

Three cassava accessions (Agric-Sé, Hombètè, Ouémènou) were examined in three different media with different combinations of growth regulators, NAA, 2,4-D (auxins), BAP, Kinetin (cytokinins), and gibberellic acid. Parameters evaluated in thermotherapy conditions were the presence of viral symptom and nodes formation. The response of explants was evaluated by aptitude to regeneration, and stems formation. The ANOVA analysis showed no significant (P˃0.05)accessions' reactions to thermotherapy, but significant (P˂0.05)accessions' response in deferent media. Thermotherapy revealed a total absence of viral symptom on the cuttings. Highest rate survival was recorded for Ouémènou accession (26 in 30) with combinations MS+NAA+KIN+GA3 and MS+2,4D+GA3.

The combination MS+NAA+BAP+GA3 made regenerate stems for all the accessions with a maximum of 14 in compared to the 30 regenerated by the Ouémènou accession.