Bacteriological Investigation of the Infectious Risks in a Semi-Public

Abstract

Biomedical analysis laboratories represent a particular sector of healthcare systems whereby professionals are especially exposed to high infectious risks. This study assessed the level of hygiene in the biomedical laboratory of a regional semi-public hospital from May 18th to August 18th, 2015. A checklist of good laboratory practices was set based on the laboratory inspection checklist of the World Health Organisation. The laboratory was divided into two sub-sections A1 and A2. 91 swabs were collected from the two sections. All these samples were then subjected to bacteriological analysis. Hygiene was less observed in section A1 than section A2. Similarly, the framework, the wastes management and technical arrangements are in contradiction with biosecurity rules. After incubation, 55 samples were infected. Coagulase negative Staphylococcus (57.58%) were the most commonly isolated bacteria from hands. Cell phones were essentially contaminated by Pseudomonas aeruginosa, Enterobacter cloaceae, Klebsiella pneumoniae and Acinetobacter spp in equal proportions of 25%. Escherichia coli were the most isolated bacteria from work surfaces (83.33%). All door knobs were contaminated by Escherichia coli (100%). Almost all these isolated bacteria were multidrug resistant. Due to the importance of laboratories in the sanitary system of hospitals, urgent and appropriate measures must be taken to mitigate the risk of infections among professionals.