Pancreatic beta cells persistently infected with coxsackievirus B4 are targets of NK cell-mediated cytolytic activity

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AuteurNEKOUA, P MAGLOIRE
AuteurBERTIN, ANTOINE
AuteurSANE, FAMARA
AuteurALIDJINOU, ENAGNON KAZALI
AuteurLOBERT, DELPHINE
AuteurTRAUET, JACQUES
AuteurHOBER, CHRISTINE
AuteurENGELMANN, ILKA
AuteurMOUTAIROU, KABIROU
AuteurYESSOUFOU, AKADIRI A RACHIDI
AuteurHOBER, DIDIER
Date d'ajout2026-06-02T16:06:57Z
Date de disponibilite2026-06-02T16:06:57Z
Date de publication2020
ResumeIt has been suggested that the persistence of coxsackieviruses-B (CV-B) in pancreatic beta cells plays a role in the pathogenesis of type 1 diabetes (T1D). Yet, immunological effectors, especially natural killer (NK) cells, are supposed to clear virus-infected cells. Therefore, an evaluation of the response of NK cells to pancreatic beta cells persistently infected with CV-B4 was conducted. A persistent CV-B4 infection was established in 1.1B4 pancreatic beta cells. Infectious particles were found in supernatants throughout the culture period. The proportion of cells containing viral protein VP1 was low (< 5%), although a large proportion of cells harbored viral RNA (around 50%), whilst cell viability was preserved. HLA class I cell surface expression was downregulated in persistently infected cultures, but HLA class I mRNA levels were unchanged in comparison with mock-infected cells. The cytolytic activities of IL-2-activated non-adherent peripheral blood mononuclear cells (PBMCs) and of NK cells were higher towards persistently infected cells than towards mock-infected cells, as assessed by an LDH release assay. Impaired cytolytic activity of IL-2-activated non-adherent PBMCs from patients with T1D towards infected beta cells was observed. In conclusion, pancreatic beta cells persistently infected with CV-B4 can be lysed by NK cells, implying that impaired cytolytic activity of these effector cells may play a role in the persistence of CV-B in the host and thus in the viral pathogenesis of T1D.
DOI10.1007/s00018-019-03168-4
Autre identifiantBECDB-8563
URIhttps://dspace.uac.bj/handle/123456789/7684
Languefr
Fait partie deCellular and Molecular Life Sciences
SujetIt has been suggested that the persistence of coxsackieviruses-B (CV-B) in pancreatic beta cells plays a role in the pathogenesis of type 1 diabetes (T1D). Yet
Sujetimmunological effectors
Sujetespecially natural killer (NK) cells
Sujetare supposed to clear virus-inf
TitrePancreatic beta cells persistently infected with coxsackievirus B4 are targets of NK cell-mediated cytolytic activity
TypeArticle

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