Micriopropagation Of Two Cultivavars of Taro [Colocasia esculenta (L.) Schott] Produced In Benin by Direct Organogenesis. SAJ Biotechnol

Abstract

Taro [Colocasia esculenta (L.) Schott] is a very nutritious plant. It is traditionally multiplied. This production strategy is a route for the dissemination of microorganisms. It is essential to foresee the path of in vitro micropropagation. This study aims to determine the effect of hormonal combinations of naphthalene acetic acid (0.5 mg / L; 1 mg / L) and benzylamino purine (2 mg / L; 4 mg / L) on micropropagation and in vitro organogenesis from apical explants of pink-fleshed and white-fleshed taro cultivars produced in Benin. A logistic regression was performed to understand the effect of different doses of naphthalene acetic acid, benzylamino purine and their combination on the apical buds of taro. The results showed that the combination of 0.5 mg / L of naphthalene acetic acid with 4 mg / L of benzylamino purine promoted the formation of the highest average number of roots in both cultivars. In relation to the number of leaves, the combination MS + 4 mg / L BAP + 0.5 mg / L ANA was more suitable for leaf formation in the white-fleshed cultivar while the combination MS + 2 mgl- 1 BAP + 1 mgl-1 ANA was more suitable for leaf formation in the pink-fleshed cultivar. On the other hand, the combination of 0.5 mg / L of naphthalene acetic acid and 2 mg / L of benzylamino purine favored the highest average number of suckers (3) in the pink-fleshed cultivar whereas in the cultivar with white flesh it was the combination of 0.5 mg / L of naphthalene acetic acid and 4 mg / L of benzylamino purine that favored the formation of the highest average number of rejections (5). The present study, realizing the success of healthy seed production, offers the opportunity of in vitro conservation in order to limit the risks of contamination and increase the production of taro in Benin.