A simple and efficient genomic DNA extraction protocol for large scale genetic analyses of plant biological systems

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AuteurCHABI SIKA, KAMIROU
AuteurKEFELA, T
AuteurADOUKONOU, AWO SAGBADJA HUBERT
AuteurAHOTON, ESSEHOU LEONARD
AuteurSAIDOU, ABDOULKARIMOU BDOUKARIMOU
AuteurBABA MOUSSA, LAMINE SAID
AuteurBAPTISTE, L J
AuteurKOTCHONI, SIMEON O
AuteurGACHOMO, EMMA W
Date d'ajout2026-06-02T16:06:57Z
Date de disponibilite2026-06-02T16:06:57Z
Date de publication2015
ResumeExtraction of high quality genomic DNA fromhigher plants is hindered by the presence of secondary metabolites, which reduce the yield and quality of the DNA. We describe an alternative protocol for genomic DNA extraction from fresh and dry plant leaves that is amenable to PCR-based genetic analysis. Existing methods were either very lengthy, expensive or not suitable for extraction of genomic DNA from dry leaves. Our method used SDS and high salt concentrations to extract DNA and does not require use of hazardous materials or special laboratory equipment. Genomic DNA extracted using our method was used for PCR-based genetic characterization of different varieties of cashew trees, Anacardium occidentale, via SSR markers as well as Zea mays varieties. This protocol improves existing methods in that it has the advantage of being adaptable to studies with a large number of samples and limited resources. The method is rapid, cost efficient and uses non-hazardous reagents. Genomic DNA extracted using this method has sufficient quality for downstream PCR-based genetic analysis.
DOI10.1016/j.plgene.2015.03.001
Autre identifiantBECDB-785
URIhttps://dspace.uac.bj/handle/123456789/1116
Languefr
Fait partie dePlant Gene
SujetDNA extraction
SujetDried leaf
SujetCashew
SujetMaize
SujetPCR
SujetSSR markers
TitreA simple and efficient genomic DNA extraction protocol for large scale genetic analyses of plant biological systems
TypeArticle

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