Densitometric HPTLC Quantification of 2-Azaanthraquinone Isolated from Mitracarpus scaber and Antimicrobial Activity against Dermatophilus congolensis

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AuteurGBAGUIDI, AHOKANNOU FERNAND
AuteurMUCCIOLI, GIULIO G
AuteurACCROMBESSI, GEORGES C
AuteurMOUDACHIROU, MANSOUROU
AuteurQUETIN LECLERCQ, JOELLE
Date d'ajout2026-06-02T16:06:57Z
Date de disponibilite2026-06-02T16:06:57Z
Date de publication2005
Resume2-Azaanthraquinone (benzo[g]isoquinoline) isolated from Mitracarpus scaber has been found to have in-vitro antimicrobial activity against Dermatophilus congolensis (MIC 7.5 µg mL–1), the causative agent of bovine dermatophilosis. To quantify this compound in Mitracarpus scaber, a new, simple and rapid high-performance thinlayer chromatographic (HPTLC) method was developed and validated for selectivity, recovery, and repeatability. Compounds were separated on silica gel 60F254 plates with toluene–ethyl acetate– methanol, 80 + 18 + 2, as mobile phase. Detection was performed by densitometric scanning at λ = 310 nm and calibration plots showed the response was linearly dependent on concentration in the range 10–100 µg mL–1, with good values of R2. The method was repeatable and precise with relative standard deviations between 0.98 and 1.59% intra-day and between 3.41 and 5.56% inter-day for concentrations from 10 to 100 µg mL–1. Limits of detection and quantification were 3 and 6 µg mL–1, respectively (corresponding to 15 and 30 ng, respectively, on the plate). Concentrations of azaanthraquinone were found to be 0.009% in Mitracarpus scaber aerial parts, 0.057% in the alcoholic extract, and 1.95% in the alkaloid extract. Azaanthraquinone may explain the antimicrobial activity of this last extract and part of the effect of the alcoholic extract.
Autre identifiantBECDB-1680
URIhttps://dspace.uac.bj/handle/123456789/1814
Languefr
Fait partie deJournal of Planar Chromatography
SujetHPTLC
SujetMitracarpus scaber
SujetDermatophilus congolensis
Sujet2-Azaanthraquinone
TitreDensitometric HPTLC Quantification of 2-Azaanthraquinone Isolated from Mitracarpus scaber and Antimicrobial Activity against Dermatophilus congolensis
TypeArticle

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