a-Glucosidase inhibition, antioxidant and cytotoxicity activities of semiethanolicextracts of Bridellia ferruginea benth. and Ceiba pentandraL. Gaerth from Benin

Abstract

The use of plant extracts in the treatment of human disease requires a definition of optimal conditions. This study objective is to evaluate the inhibition capacity of
-glucosidase activity of Bridelia ferruginea benth bark and Ceiba pentandra L. Gaerth root semi-ethanolic extracts compared to acarbose. Their antioxidant activity were also tested by three techniques: 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, Ferric reducing antioxidant power (FRAP) and the Oxygen Radical Absorbance Capacity (ORAC). Results indicated that these extracts have antioxidant property and
-Glucosidase inhibitory activity (IC50 = 1.4 ± 0.04 μg / mL for B. ferruginea and 51 ± 0.7 μg / mL for C. pentandra) higher than the reference compound acarbose (IC50 = 726 ± 15 mg / mL). Bridelia ferruginea extract was the most active of the two. In vitro cytotoxicity evaluation of the extracts was done by fluorometric assay: Resazurin reduction test on human fibroblast primary culture have showed a very low toxicity. Bridelia ferruginea and Ceiba pentandra The semi-ethanolic extracts could therefore constitute a credible alternative to replace the expensive synthetic drugs in the treatment of diabetes.