Influence of Explants Type, Nodal Positions and Two Cytokinins on Cassava (Manihot esculenta, Crantz) In vitro Organogenesis

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AuteurCACAI, GILLES HABIB TODJRO
Date d'ajout2026-06-02T16:06:57Z
Date de disponibilite2026-06-02T16:06:57Z
Date de publication2023
ResumeCassava In vitro regeneration is essentially influenced by exogen factors as well as explants nature. This work aimed to evaluate explants type and positions influence, phytohormone on cassava plantlets regeneration in modified MS media. The apex and nodes of the position 3, 5, and 7 were initiated on Murashige and Skoog (MS) based media supplemented with Gamborg B5 vitamins and growth regulators such as naphthalene acetic acid (NAA), 6-benzylaminopurine (BAP) and zeatin (ZEAT) in different concentrations. The results indicated a significant difference (p=0.0001) among explants sprouted regarding the nodes position and the cultivar genotype during the time of culture. The highest rate of regenerated plantlets was found with nodes of position 5 (95.33%) followed respectively by nodes of the position 7 (93.33%), nodes of the position 3 (89.33%), and the apex (76%). Regarding the growth regulators, NAA (0.5 mg/l) + BAP (0.5 mg/l) combination was favorable for height growth (26.2cm), and leaves development (7.84 cm) in most of cultivars, whereas NAA (0.5 mg/l) + ZEAT (0.5 mg/l) combination was more favorable to rhizogenesis in overall cultivars. Among the cultivars, the best performance in stem height (26.2 cm), in leaf length (7.84 cm), and roots number (4.64) were observed with INA 1er cultivar. The cultivars RB89509, BEN 86052, and DOKU were less grown on MS supplemented with 0.5 mg/l of BAP or ZEAT. These findings could contribute of using nodes of the positions 5, 7 as potential explants and Zeatin for cassava plantlets rhizogenesis
DOI10.9734/JABB/2023/v26i12669
Autre identifiantBECDB-13154
URIhttps://dspace.uac.bj/handle/123456789/11303
Languefr
Fait partie deJournal of Advances in Biology & Biotechnology
SujetCassava micropropagation
SujetIn vitro culture
Sujetnature of explant
Sujetgrowth regulators
Sujetplantlets
Sujetrégénération
TitreInfluence of Explants Type, Nodal Positions and Two Cytokinins on Cassava (Manihot esculenta, Crantz) In vitro Organogenesis
TypeArticle

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