Effet du charbon actif sur la conservation de la variété de manioc (Manihot esculenta) RB 89509 menacée d’extinction au Bénin

Abstract

In situ conservation of different cultivars of cassava (Manihot esculenta) implies many problems such as climatic risks, diseases, devastators and financial difficulties. This work contributes to the maintenance of RB 89509 variety of Manihot esculenta through in vitro culture. Two cultures media were used: medium of Murashige and Skoog (MS) and medium of Murashige and Skoog (MS) added with activated carbon. The effects of activated carbon were studied on the growth parameters (number of leaves, number of roots, number of nodes, height of stem, length of internodes, length of principal root) of plantlets aged of twenty months. The results show that the average number of leaves, the average number of nodes and the length of main root are more significant at the plantlets present on medium containing the activated carbon; while the number of roots, the height of stem and the length of internodes are more significant at the plantlets present on medium without activated carbon. Moreover, it is noted an drying of the plantlets on the medium without activated carbon. The activated carbon allowed a better conservation of the plantlets.